Protocols

Biochemical assays  Clinical Research  Cell Culture  Antibody Methods
 
 
Below is a list of the major protocols used by members of the group. These are available both as full text pages and downloadable as pdf documents for easy printing. Please note that all of our protocols are provided for information only, in the hope that you may find them useful in your research and all of them are subject to our standard disclaimer.

Protocols for the use of antibodies

 

IMMUNOFLUORESENCE STAINING (including QIF) :: Version 1.0

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IMMUNOPRECIPITATION(general protocol) :: Version 1.0

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ELISA to quantitate mouse IgG :: Version 1.0

 

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Protocols for biochemical assays

 

PREPARATION OF WASHED BLOD CLOTS :: Version 1.0

 

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Protocols related to clinical research and epidemiology

 

PREPARATION OF PLASMA WITH MINIMAL PLATELET DEGRANULATION FOR TGFb ASSAYS:: Version 1.0

 

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Protocols related to the culture of mammalian cells and assays of cell function

 

TRANSWELL FILTER MIGRATION ASSAY:: Version 1.0

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COLLAGEN BIOSYNTHESIS: [3H]-proline incorporation:: Version 1.0

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PRIMARY CULTURE OF RAT VASCULAR SMOOTH MUSCLE CELLS:: Version 1.0

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COUNTING VASCULAR SMOOTH MUSCLE CELLS:: Version 1.0